Application

High Sensitivity NADPH Assay Kit has been used to determine the intracellular NAD(P)H and NAD(P)⁺ levels.

General description

The reduced form of nicotinamide dinucleotide (NADPH) functions as the cofactor for many redox enzymes and contributes to reductive biosynthetic pathways. NADPH is mainly produced from glucose through the oxidative pentose phosphate pathway.

Principle

The High Sensitivity NADPH Quantification Fluorometric Assay Kit is suitable for quantifying NADPH (ranging from 2-10 pmole/well) in tissues, cells, and mitochondria. The kit detects intracellular NAPDH in the enzyme recycling reaction and does not require purified NADPH from the samples. NADPH is determined by measuring a fluorescent product (λ_ex = 535/ λ_em = 587 nm) proportional to the amount of NADPH present.

Suitability

Suitable for the detection of NADPH in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.